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  5. Volume 1, Issue 2 (2013)
  6. Modeling amperometric biosensors based o ...

Computational Science and Techniques

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Modeling amperometric biosensors based on allosteric enzymes
Volume 1, Issue 2 (2013), pp. 205–213
Liutauras Ričkus  

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Pub. online: 18 September 2013      Type: Article      Open accessOpen Access

Received
22 July 2013
Accepted
21 August 2013
Published
18 September 2013

Abstract

Computational modeling of a biosensor with allosteric enzyme layer was investigated in this study. The operation of the biosensor is modeled using non-stationary reaction-diffusion equations. The model involves three regions: the allosteric enzyme layer where the allosteric enzyme reactions as well as then mass transport by diffusion take place, the diffusion region where the mass transport by diffusion and non-enzymatic reactions take place and the convective region in which the analyte concentration is maintained constant. The biosensor response on dependency substrate concentration, cooperativity coefficient and the diffusion layer thickness on the same parameters have been studied.

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