Unlike the war veterans or disabled soldiers’ associations that were active in Lithuania in the period between the two world wars, which have already been extensively studied, less is known about associations that provided public relief to the army. One of them supported the notion of women’s involvement in national defence, which was widespread in European society in the 19th century and the first half of the 20th century. Named in 1935 after the mother of Lithuania’s greatest Medieval ruler, it was called the Duchess Birutė Association of Women from Officers’ Families. Based on research into existing sources and literature, the article sets out to analyse the establishment of this association, which was active for 15 years (1925–1940), and to reveal its aims and structure. The author examines the statutes which defined the directions of its activities and financial possibilities, identifies sponsors, and assesses their impact on the operation of the association, before focusing on the activities of the association relating to cooperation with the army, the dissemination of national ideas, and the concepts of family and the role of women.