Research problem: The study addresses how the application of learning techniques influences students’ acquisition of biological knowledge, and what possibilities exist for their implementation in classroom settings. Research aim: To reveal how learning techniques affect students’ knowledge acquisition and application. Research object: The acquisition of biological knowledge through the application of learning techniques. Research implementation: The study was conducted during the second semester of the 2025 academic year, from January to May, involving students from the 9th to the 12th grades on a gymnasium biology course. The initial analysis revealed that students found it easier to memorise concepts and choose correct answers, while performing more complex, real-life context tasks proved to be more challenging. To enhance knowledge acquisition, active learning techniques were applied in the lessons, including mnemonics, meaningful repetition, and deliberate memorisation. Research results: The findings revealed that the applied learning techniques improved the understanding of biological concepts and phenomena, facilitated the application of knowledge in new situations, strengthened analytical thinking skills, and encouraged active participation in discussions. The application of learning techniques contributed to the deepening of biological knowledge, the strengthening of cognitive competence, and the long-term retention of knowledge in biology lessons.