The article is devoted to the history of Vilnius evangelics in the 20th Century. The Vilnius evangelics played a significant role on the Polish protestant map in the interwar period. For the Lutherans inhabiting the North-Eastern area of Poland, Vilnius was the capital of their diocese. In turn, the Calvinists had their own Vilnius Church. It steadily developed throughout the interwar period founding its branches in central Poland. The Vilnius Calvinists never forgot about the tradition of the Grand Duchy of Lithuania, keeping contact with the church in the Lithuanian Republic. The custom of Polish and Lithuanian Evangelics’ cooperation was returned to after regaining independence in 1989, which is exemplified by the cooperation of Lutheran Polish and Lithuanian churches.