The authors discuss the archaeozoological indicators for horse domestication, and come to a conclusion that a considerable increase of horse remains, accompanied by a presence of other certainly domesticated species could be one of them. With such a situation we have to do in Ayakagytma ‘The Site’, Uzbekistan, where in the Early Neolithic layers dated to 8000–7400 cal. BP, a share of horse remains reach 30–40%. It would suggest the earliest horse domestication known today.