This article discusses issues related to pile-dwelling settlements in Lithuania. It offers a detailed study of the archaeological and osteological material found at the Žemaitiškė 2 pile-dwelling settlement, as well as palynological and radiocarbon research into the settlement’s cultural layer. The article discusses the wood anatomy of pile-dwellings, their dendrochronological dating, and the types of construction material. The studies show that the construction of pile-dwellings in Lithuania began in the Late Neolithic Age, whereas the tradition of living on pile platforms existed throughout the Bronze Age.